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±¸°­³» ¹Ì»ý¹°¿¡ ´ëÇÑ ¼­¾ç»ê °íÃß³ÃÀÌ(Armoracia rusticana) »Ñ¸® ÃßÃâ¹°ÀÇ Ç×±ÕÈ¿°ú

THE ANTIMICROBIAL EFFECT OF HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AGAINST ORAL PATHOGENS

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ÀÌÁÖÇö ( Lee Ju-Hyun ) - °­¸ª´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç ¹× ±¸°­°úÇבּ¸¼Ò
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Abstract

¼­¾ç»ê °íÃß³ÃÀÌ (Armoracia rusticara)´Â Çâ½Å·á·Î¼­ÀÇ ¿ªÇÒ»Ó¸¸ ¾Æ´Ï¶ó Ç×±Õ È¿°ú·Îµµ ÁÖ¸ñÀ» ¹Þ°í ÀÖ´Ù. °íÃß³ÃÀÌÀÇ Ç×±Õ¼ººÐÀº isothiocyanate·ù·Î ±× Áß¿¡¼­µµ allyl isothiocyanate(AIT)°¡ Ç×±ÕÈ°¼ºÀÌ °­ÇÑ °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. º» ¿¬±¸¿¡¼­´Â ÃÖ¼Ò¾ïÁ¦³óµµ¿Í ÃÖ¼Ò»ì±Õ³óµµ¸¦ ÃøÁ¤ÇÏ¿© ´Ù¾çÇÑ ±¸°­³» ¹Ì»ý¹°(Streptococcus mutans ATCC 25175, Streptococcus sobrinus(d) ATCC 27607 Lactobacillus casei ATCC 393, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Actinobacillus actimomycetemcomitans ATCC 29522, Candida albicans ATCC 10261)¿¡ ´ëÇÑ ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°ÀÇ Ç×±ÕÈ¿°ú¸¦ ¾Ë¾Æº¸°í, À̸¦ ´ëÇ¥Àû Ç×±ÕÁ¦ÀΠŬ·Î¸£Çí½ÃµòÀÇ Ç×±ÕÈ°¼º°ú ºñ±³ÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
1. ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°Àº ¿¬±¸¿¡ »ç¿ëµÈ 7Á¾ÀÇ ±¸°­³» ¹Ì»ý¹°¿¡ ´ëÇØ ÃÖ¼Ò¾ïÁ¦³óµµ´Â 30-125ppm, ÃÖ¼Ò»ì±Õ³óµµ´Â 125-500ppmÀ» ³ªÅ¸³ÂÀ¸¸ç, ƯÈ÷ ¼¼±Õº¸´Ù´Â È¿¸ðÀÎ C. albicans¿¡ ´ëÇØ °­ÇÑ Ç×±ÕÈ°¼ºÀ» ³ªÅ¸³½´Ù.
2. Ŭ·Î¸£Çí½Ãµò ¿ª½Ã 7Á¾ÀÇ ±¸°­³» ¹Ì»ý¹°¿¡ ´ëÇØ Ç×±ÕÈ¿°ú¸¦ ³ªÅ¸³ÂÀ¸¸ç, ÃÖ¼Ò¾ïÁ¦³óµµ´Â 0.15-2.5% ÃÖ¼Ò»ì±Õ³óµµ´Â 0.4-2.5%·Î ÃøÁ¤µÇ¾ú´Ù.
ÀÌ»óÀÇ °á°ú·Î ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°Àº Ŭ·Î¸£Çí½Ãµò°ú ºñ±³ÇÏ¿© ´õ ³·Àº ³óµµ¿¡¼­ Ŭ·Î¸£Çí½Ãµò°ú À¯»çÇÑ Á¤µµÀÇ Ç×±ÕÈ¿°ú¸¦ ³ªÅ¸³¿À» ¾Ë ¼ö ÀÖ´Ù.

In this study, the antimicrobial effects of Horseradish (Armoracia rusticana) root extracts against oral pathogens were investigated, and also compared with that of chlorhexidine. The following 7 microorganisms were used in this study, Streptococcus mutans ATCC 25175, Streptococcus sobrinus(d) ATCC 27607, Lactobacillus casei ATCC 393, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Actinobacillus actinomycetemcomitans ATCC 29522. Candida albicans ATCC 10261. Horseradish root extracts and chlorhexidine were tested to determine their minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC).
The results of this study can be summarized as follows :
1. Horseradish root extracts showed antimicrobial effect against the tested oral pathogens. MIC and MBC of this extracts were 30-125, 125-500ppm, respectively. Especially, it was the most effective against C. albicans of other tested microorganisms.
2. Chlorhexidine also showed antimicrobial effect against the tested oral pathogens. MIC of chlorhexidine range between 0.15 and 2.5%, MBC are 0.4-2.5%.
In conclusion, it was suggested that AIT had similar antimicrobial effects in the lower concentration, compared with that of chlorhexidine.

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¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°;Ŭ·Î¸£Çí½Ãµò;Ç×±ÕÈ¿°ú
Horseradish(Armoracia rusticana) root extracts;Allyl isothiocyanate(AIT);Chlorhexidine;Antimicrobial effect

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